Acta Entomologica Serbica http://aes.bio.bg.ac.rs/index.php/aes Acta entomologica Serbica is a peer-reviewed scientific journal covering entomology published by the Entomological Society of Serbia. It publishes mainly original research papers. ENTOMOLOGICAL SOCIETY OF SERBIA en-US Acta Entomologica Serbica 0354-9410 <p><strong>Copyright:</strong>&nbsp;© 2019 The ENTOMOLOGICAL SOCIETY OF SERBIA Staff. This is an open-access article distributed under the terms of the&nbsp;<a href="http://creativecommons.org/licenses/by/4.0/">Creative Commons Attribution License</a>, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.</p> INSECTICIDAL AND ANTIOXIDANT ACTIVITIES OF AQUEOUS EXTRACTS OF TWO ALGERIAN MEDICINAL PLANTS http://aes.bio.bg.ac.rs/index.php/aes/article/view/258 <p>In this work, we determined the insecticidal activity and antioxidant activity of aqueous extracts of <em>Nerium oleander</em> (Apocynaceae) leaves and <em>Peganum harmala</em> (Zygophyllaceae) seeds, two well-known Algerian medicinal plants. Four concentrations of each extract were applied in total randomization by spraying directly on the eggs and larvae of <em>Ectomyelois ceratoniae</em> (Zeller, 1879) (Lepidoptera: Pyralidae) under laboratory conditions. Our findings revealed that eggs hatching after four days were not affected by the aqueous extract, with a rate of 54% of hatching eggs in both control and treated eggs. Additionally, toxicity by contact on larvae was not evident and the mortality percentage did not exceed 8%, regardless of the extract plant and concentration. Phytochemical screening showed a slight presence of terpenes and saponins and an absence of alkaloids. However, polyphenols were present in medium concentrations in the aqueous extracts of both plants. The antioxidant activities of <em>N. oleander</em> and <em>P. harmala</em> extracts were found to be 71.80 and 72.94%, respectively. Total phenolic content (TPC) and total flavonoid content (TFC) of the studied extracts were 50.69 mg gallic acid equivalent (GAE)/100 g dry weight (DW) and 3.55 in terms of quercetin equivalent (QE)/100 g DW, respectively, in <em>N. oleander</em>. In <em>P. harmala</em>, the TPC and TFC were 52.30 mg GAE/100 g DW and 3.49 mg QE/100 g DW, respectively. Our study clearly shows the limited insecticidal effect of the aqueous extracts, although they showed an interesting antioxidant potential, which could help to decrease the incidence of oxidative stress-induced damage.</p> Billal Nia Adel Lekbir Mohamed Kamel Ben Salah ##submission.copyrightStatement## http://creativecommons.org/licenses/by-nc-sa/4.0 2020-02-10 2020-02-10 25 1 10.5281/zenodo.3660988 NINE SPECIES OF APHIDS (HEMIPTERA: APHIDIDAE) NEW TO THE FAUNA OF SERBIA http://aes.bio.bg.ac.rs/index.php/aes/article/view/269 <p>Nine aphid species (Hemiptera: Aphididae), mainly found in the southeastern part of the country, were determined as new records for Serbia. They belong to three subfamilies: <em>Aphis passeriniana</em> (Del Guercio, 1900); <em>A. podagrariae</em> Schrank, 1801; <em>A. salviae</em> Walker, 1852; <em>Brachyunguis tamaricis</em> (Lichtenstein, 1885); <em>Smiela fusca</em> Mordvilko, 1948; <em>Staticobium limonii</em> Contarini, 1847 and <em>Uroleucon pseudobscurum</em> (Hille Ris Lambers, 1967) from the subfamily Aphidinae; <em>Drepanosiphum oregonensis</em> Granovsky, 1939 from the subfamily Drepanosiphinae and <em>Pterocomma jacksoni</em> Theobald, 1921 from the subfamily Pterocommatinae. With the inclusion of these nine records, a total of 383 aphids have been found so far in Serbia. This represents about 70% of the species of the total aphid fauna expected to be found on plants in Serbia. All species are of Palearctic distribution and seem to be monoecious.</p> Olivera Petrović Obradović Marijana Ilić Milošević Saša S. Stanković Vladimir A Žikić ##submission.copyrightStatement## http://creativecommons.org/licenses/by-nc-sa/4.0 2020-03-06 2020-03-06 25 1 10.5281/zenodo.3699562 THREE MONTANE GRASS MOTHS (LEPIDOPTERA: CRAMBIDAE) NEW TO THE FAUNA OF CROATIA http://aes.bio.bg.ac.rs/index.php/aes/article/view/265 <p>Three species of grass moths (Crambidae) were recorded for the first time in the country. <em>Udea alpinalis</em> and <em>Crambus ericella </em>were recorded on the peaks Guslica and Snježnik in Gorski kotar region, while <em>Catoptria pyramidellus </em>was recorded on several different mountain chains across the country. The record of three montane species in Croatia shows the lack of Microlepidoptera surveys and the need for further visits to those areas.</p> Toni Koren ##submission.copyrightStatement## http://creativecommons.org/licenses/by-nc-sa/4.0 2020-03-31 2020-03-31 25 1 10.5281/zenodo.3735368 EFFECT OF DIFFERENT LIGHT SPECTRA ON FECUNDITY OF EPHESTIA KUEHNIELLA ZELLER (LEPIDOPTERA: PYRALIDAE) http://aes.bio.bg.ac.rs/index.php/aes/article/view/268 <p>This paper examines the effects of different light spectra on the number of eggs of <em>Ephestia kuehniella </em>Zeller (Lepidoptera: Pyralidae) as a general host of biological control agents. Six distinct spectra of light, ranging from 395 nm to 625 nm, were examined in laboratory conditions. Green and red spectra with ranges of 520-525 nm and 620-625 nm caused the highest and the lowest numbers of eggs, respectively. Additionally, the effects of time on the mean egg load of <em>E. kuehniella</em> were also found to be significant at five days. The implications of the current results are discussed below and suggest that the application of a green spectrum in insectariums could help in increasing egg production.</p> Fatemeh Farsi Ahmad Ashouri Seyed Hossein GOLDANSAZ ##submission.copyrightStatement## http://creativecommons.org/licenses/by-nc-sa/4.0 2020-04-02 2020-04-02 25 1 10.5281/zenodo.3737858 NEW DATA ON THE DISTRIBUTION OF PHLOEMO-XYLOPHAGOUS INSECTS OF SMOOTH-LEAVED ELM (ULMUS MINOR MILL.) AND THEIR PARASITOIDS AND PREDATORS IN SERBIA http://aes.bio.bg.ac.rs/index.php/aes/article/view/270 <p>In a study of the fauna of phloemo-xylophagous insects on <em>Ulmus minor</em> and their parasitoids and predators in Serbia, new data were obtained on the distribution of the recorded species. Some of these data have been published, but many have not. These unpublished data are presented in this paper, which contains a list of 45 species from two orders, &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;13 families and 41 genera (16 phloemo-xylophagous species, 24 species of parasitoids and five species of predators). For each of them, data are presented regarding the place and time of their finding.</p> Čedomir Marković Aleksandar Stojanović ##submission.copyrightStatement## http://creativecommons.org/licenses/by-nc-sa/4.0 2020-04-25 2020-04-25 25 1 10.5281/zenodo.3765876 DISTRIBUTION OF THE GENUS GASTERUPTION LATREILLE (HYMENOPTERA: EVANIOIDEA: GASTERUPTIIDAE) IN TURKEY http://aes.bio.bg.ac.rs/index.php/aes/article/view/275 <p>The present study is based on gasteruptiid samples <em>collected in various parts of Turkey since the 1990s. Together with literature sources, a total of 36 species of the genus </em><em>Gasteruption </em>Latreille, 1797 <em>are compiled for the country.</em> In addition, the European species <em>Gasteruption boreale </em>(Thomson, 1883) is newly recorded for Turkey and Asia. Moreover, the recently described<em> G. brevibasale</em> van Achterberg &amp; Saure, 2014, <em>G. ischnolaimum</em> van Achterberg, 2014, and &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;<em>G.</em> <em>smitorum</em> van Achterberg, 2014, are recorded for the first time from Erzurum after the original description. <em>Gasteruption henseni </em>van Achterberg, 2014 is, together with the abovementioned four species, confined to eastern Anatolia. <em>Gasteruption hastator </em>(Fabricius, 1804)<em>, G. merceti </em>Kieffer, 1904 and <em>G. tournieri</em> Schletterer, 1885, are found to be the most widespread and abundant species throughout Turkey. On the contrary, <em>G. aciculatum</em> van Achterberg, 2014, <em>G. boreale </em>and <em>G. nigritarse</em> (Thomson, 1883) are so far rarely collected in Turkey, recorded from one province each. The first was described after a single female from Van and apart from the holotype no other specimens are known. The eastern part of Turkey proved to be an important center of diversity for <em>Gasteruption</em> species. <em>Distribution maps are presented for</em> <em>the indigenous, rarely, </em>sparsely, widely and frequently recorded species.</p> Hikmet Özbek Cornelis van Achterberg ##submission.copyrightStatement## http://creativecommons.org/licenses/by-nc-sa/4.0 2020-05-28 2020-05-28 25 1 10.5281/zenodo.3862658 PHYLOGENETIC ANALYSIS OF RED COTTON BUG SPECIES (HEMIPTERA: PYRRHOCORIDAE) IN PUNJAB, PAKISTAN http://aes.bio.bg.ac.rs/index.php/aes/article/view/276 <p>Traditional techniques for identifying the red cotton bugs <em>Dysdercus koenigii </em>and<em> Dysdercus cingulatus </em>(Pyrrhocoridae: Hemiptera) depend on phenotypic and morphological data. These methods are suitable when species have well-defined structures and when huge taxonomic knowledge is at hand. In this study, the mitochondrial cytochrome oxidase I (COI) gene was selected for the molecular identification of <em>D. koenigii </em>and<em> D. cingulatus.</em> The sequence data showed that one species of the red cotton bug sample was identified as <em>Dysdercus</em><em> koenigii</em> with 35-100% similarity to other <em>Dysdercus</em> species present in the NCBI database, while the other species was identified as <em>Dysdercus cingulatus</em> with 88-94% similarity to other red cotton bugs species present in the NCBI database. The phylogenetic analysis revealed that our &nbsp;&nbsp;&nbsp;&nbsp;<em>D. koenigii </em>Seq (&gt;180319003-A02-102-DK-.ab1) shares the same cluster with four <em>D. koenigii</em> isolates (ZSI/SRC_I.28B, ZSI/SRC_I.28, GQ306227.1, KJ459924.1). While the <em>D. cingulatus</em> Seq (&gt;180319003-A02-103-DC-.abl) shares a cluster with <em>D. cingulatus voucher</em> (RO_DC2015). This is the first molecular identification of red cotton bugs (<em>D. koenigii </em>and<em> &nbsp;&nbsp;&nbsp;&nbsp;D. cingulatus</em>) reported in Pakistan. Thus, in the current study, molecular identification has been accepted as a reliable method for the identification of these agriculturally important insect pests.</p> Waqar Ali Khan Safi Ur Rehman Qamar Jam Nazeer Ahmad Mayer L Calma Asad Ullah ##submission.copyrightStatement## http://creativecommons.org/licenses/by-nc-sa/4.0 2020-06-11 2020-06-11 25 1 10.5281/zenodo.3889728 CHEILOSIA HERCULANA BRĂDESCU, 1982, A NEW HOVERFLY (DIPTERA: SYRPHIDAE) SPECIES FOR THE FAUNA OF SERBIA http://aes.bio.bg.ac.rs/index.php/aes/article/view/267 Mihailo Vujić ##submission.copyrightStatement## http://creativecommons.org/licenses/by-nc-sa/4.0 2020-02-10 2020-02-10 25 1 10.5281/zenodo.3661000 ACROSTERNUM HEEGERI (HETEROPTERA: PENTATOMIDAE) IN SERBIA http://aes.bio.bg.ac.rs/index.php/aes/article/view/279 <p><span class="tlid-translation translation" lang="en">The species <em>Acrosternum</em> <em>heegeri</em> Fieber, 1861 is a well-known member of the Mediterranean fauna in Europe and until recently was not recorded outside its natural range. In recent years, the presence of stable populations of the species in Budapest has been confirmed, and the first findings on the territory of Serbia are from 2013, when several overwintering adults were found under the bark of deciduous trees in Novi Sad. Since 2017, active individuals have been recorded throughout the season, for now mainly from the area of ​​Vojvodina. This bug is probably conquering new territories by transporting people and goods, but we assume that climate change is also responsible for establishing stable populations in new environments. The paper presents the findings of the species<em> A. heegeri</em> in Serbia, which were taken from the Alciphron database on insects in Serbia. <em>A. heegeri</em> is a polyphagous herbivore and has the status of an economically significant species in the Middle East because it causes damage in pistachio plantations.</span></p> Jelena Šeat Bojana Nadaždin Marko Šćiban ##submission.copyrightStatement## http://creativecommons.org/licenses/by-nc-sa/4.0 2020-06-01 2020-06-01 25 1 10.5281/zenodo.3871640 REVEALING THE PRESENCE OF THE EAST ASIAN LEAFHOPPER TAUTONEURA POLYMITUSA (HEMIPTERA: AUCHENORRHYNCHA: CICADELLIDAE: TYPHLOCYBINAE) IN SERBIA THROUGH DNA BARCODING http://aes.bio.bg.ac.rs/index.php/aes/article/view/289 <p>After the first observations of cicadas of the subfamily Typhlocybinae (Hemiptera: Auchenorrhyncha: Cicadellidae), a specific white-orange-red coloration in Zrenjanin and Melenci in 2017 and 2018, four females of the same habitus were collected in August 2019 in Belgrade and Special Nature Reserve “Deliblato Sands”. External morphology analyzes indicated that they are most similar to the East Asian invasive species present in Europe, <em>Tautoneura polymitusa</em> Oh &amp; Jung 2016. Due to the lack of sampled males, reliable species determination required the use of molecular methods. Since no nucleotide sequences of the barcoding region of the COI gene of the <em>T. polymitus</em> species are available in gene banks, specimens of males collected in Budapest at the site of the first find of the species in Europe were attached to the genetic identification. After successful multiplication and sequencing of the COI barcoding region, 100% genetic matching of the sequences of the analyzed females from Serbia and males from Hungary was determined. The obtained results confirm the presence of allochthonous cicada <em>T. rolymitus</em> in Serbia and indicate the importance of the barcoding method which in the context of biodiversity research successfully overcomes sampling problems such as small number of specimens or absence of males.</p> Andrea Kosovac Marko Šćiban Ivan Pančić Mária Tóth László Ronkay András Orosz ##submission.copyrightStatement## http://creativecommons.org/licenses/by-nc-sa/4.0 2020-06-05 2020-06-05 25 1 10.5281/zenodo.3878684