PHYLOGENETIC ANALYSIS OF RED COTTON BUG SPECIES (HEMIPTERA: PYRRHOCORIDAE) IN PUNJAB, PAKISTAN

Main Article Content

Waqar Ali Khan
http://orcid.org/0000-0001-5351-8987
Safi Ur Rehman Qamar
https://orcid.org/0000-0001-9834-2727
Jam Nazeer Ahmad
Mayer L Calma
http://orcid.org/0000-0002-3397-3090
Asad Ullah

Abstract

Traditional techniques for identifying the red cotton bugs Dysdercus koenigii and Dysdercus cingulatus (Pyrrhocoridae: Hemiptera) depend on phenotypic and morphological data. These methods are suitable when species have well-defined structures and when huge taxonomic knowledge is at hand. In this study, the mitochondrial cytochrome oxidase I (COI) gene was selected for the molecular identification of D. koenigii and D. cingulatus. The sequence data showed that one species of the red cotton bug sample was identified as Dysdercus koenigii with 35-100% similarity to other Dysdercus species present in the NCBI database, while the other species was identified as Dysdercus cingulatus with 88-94% similarity to other red cotton bugs species present in the NCBI database. The phylogenetic analysis revealed that our     D. koenigii Seq (>180319003-A02-102-DK-.ab1) shares the same cluster with four D. koenigii isolates (ZSI/SRC_I.28B, ZSI/SRC_I.28, GQ306227.1, KJ459924.1). While the D. cingulatus Seq (>180319003-A02-103-DC-.abl) shares a cluster with D. cingulatus voucher (RO_DC2015). This is the first molecular identification of red cotton bugs (D. koenigii and     D. cingulatus) reported in Pakistan. Thus, in the current study, molecular identification has been accepted as a reliable method for the identification of these agriculturally important insect pests.

Article Details

How to Cite
Khan, W. A., Qamar, S. U. R., Ahmad, J. N., Calma, M., & Ullah, A. (2020). PHYLOGENETIC ANALYSIS OF RED COTTON BUG SPECIES (HEMIPTERA: PYRRHOCORIDAE) IN PUNJAB, PAKISTAN. Acta Entomologica Serbica, 25(1). https://doi.org/10.5281/zenodo.3889728
Section
Articles

References

Ahmad, J. N., Jafir, M., Javed, M. W., Maqsood, S., & Ahmad, S. J. (2019). Molecular identification and sequence analysis of dusky cotton bug, Oxycarenus hyalinipennis (Hemiptera: Lygaiedae) infesting cotton field in Pakistan. Pakistan Journal of Zoology, 51(2), 789-792.

Ahmad, J. N., Mushtaq, R., Ahmad, S. J. N., Maqsood, S., Ahuja, I., & Bones, A. M. (2018). Molecular identification and pathological characteristics of NPV isolated from Spodoptera litura (Fabricius) in Pakistan. Pakistan Journal of Zoology, 50, 2229-2237.

Atwal, A., & Dhaliwal, G. (2015). Agricultural pests of South Asia and their management: Kalyani Publishers, 487 pp.

Bakhsh, K., Hassan, I., & Maqbool, A. (2005). Factors affecting cotton yield: a case study of Sargodha (Pakistan). Journal of Agriculture & Social Sciences, 1(4), 332-334.

da Silva, V. C. P., Bertin, A., Blin, A., Germain, J.-F., Bernardi, D., Rignol, G., Botton, M., & Malausa, T. (2014). Molecular and morphological identification of mealybug species (Hemiptera: Pseudococcidae) in Brazilian vineyards. PloS one, 9(7), e103267.

Folmer, O., Black, M., Hoeh, W., Lutz, R., & Vrijenhoek, R. (1994). DNA primers for amplification of mitochondrial cytochrome c oxidase subunit I from diverse metazoan invertebrates. Molecular Marine Biology and Biotechnology, 3(5), 294-299.

Gullan, P., Downie, D., & Steffan, S. (2003). A new pest species of the mealybug genus Ferrisia Fullaway (Hemiptera: Pseudococcidae) from the United States. Annals of the Entomological Society of America, 96(6), 723-737.

Habeeb, S., Kumar, S., Sanjayan, K., Anuradha, V., & Praveena, A. (2011). Comparative evolutionary analysis of insects using mitochondrial genes. International Journal of Current Research, 3(11), 66-73.

He, Y. B., Zhan, R. L., Sun, G. M., Wu, J. B., & Zhao, Y. L. (2015). Phylogeography of pink pineapple mealybugs, Dysmicoccus brevipes (Cockerell) reveals the history of pineapple introduction and cultivation in China. Genetics and molecular research: GMR, 14(3), 9890-9897. doi:10.4238/2015.August.19.23

Hebert, P. D., Cywinska, A., Ball, S. L., & Dewaard, J. R. (2003). Biological identifications through DNA barcodes. Proceedings of the Royal Society of London. Series B: Biological Sciences, 270(1512), 313-321.

Holm, L., Pancho, J. V., Herberger, J. P., & Plucknett, D. L. (1979). A geographical atlas of world weeds: John Wiley and Sons, 391 pp.

Jaleel, W., Saeed, S., Naqqash, M. N., & Zaka, S. M. (2014). Survey of Bt cotton in Punjab Pakistan related to the knowledge, perception and practices of farmers regarding insect pests. International Journal of Agriculture and Crop Sciences, 7(1), 10.

Khan, S. M., Ali, S., Nawaz, A., Bukhari, S. A. H., Ejaz, S., & Ahmad, S. (2019). Integrated pest and disease management for better agronomic crop production. In Agronomic Crops (pp. 385-428). Springer, Singapore. https://doi.org/10.1007/978-981-32-9783-8_19

Malausa, J., Blanchet, A., Bon, M., Cheyppe-Buchmann, S., Groussier-Bout, G., Jones, W., Pickett, C., Ris, N., Roche, M., & Thaon, M. (2010). Introduction of the African parasitoid Psyttalia lounsburyi in south of France for the classical biological control of Bactrocera oleae: will hybridization affect establishment and population growth? International Organization for Biological and Integrated Control of Noxious Animals and Plants (OIBC/OILB), West Palaearctic Regional Section (WPRS/SROP) Dijon France, 53, 49-55.

Mills, N. J., & Kean, J. M. (2010). Behavioral studies, molecular approaches, and modeling: methodological contributions to biological control success. Biological Control, 52(3), 255-262.

Nagoshi, R. N., Paraiso, O., Brambila, J., & Kairo, M. T. (2012). Assessing the usefulness of DNA barcoding to identify Oxycarenus hyalinipennis (Hemiptera: Oxycarenidae) in Florida, a potentially invasive pest of cotton. Florida Entomologist, 95(4), 1174-1182.

Natarajan, K., Rajendran, T., & Rathinavel, K. (2005). Pest management in cotton in DUS experimentation. DUS testing in cotton. All India Coordinated Cotton Improvement Project, Central Institute for Cotton Research Regional Station, Coimbatore, India, 124-135.

Pandey, J., & Tiwari, R. (2011). Neem based insecticides interaction with development and fecundity of red cotton bug, Dysdercus cingulatus Fab. International Journal of Agricultural Research, 6(4), 335-346.

Park, D.-S., Suh, S.-J., Hebert, P. D., Oh, H.-W., & Hong, K.-J. (2011). DNA barcodes for two scale insect families, mealybugs (Hemiptera: Pseudococcidae) and armored scales (Hemiptera: Diaspididae). Bulletin of entomological research, 101(4), 429-434.

Stouthamer, R., Hu, J., van Kan, F. J., Platner, G. R., & Pinto, J. D. (1999). The utility of internally transcribed spacer 2 DNA sequences of the nuclear ribosomal gene for distinguishing sibling species of Trichogramma. BioControl, 43(4), 421-440.

Tamura, K., & Nei, M. (1993). Estimation of the number of nucleotide substitutions in the control region of mitochondrial DNA in humans and chimpanzees. Molecular biology and evolution, 10(3), 512-526.

Wang, X.-g., & Messing, R. H. (2003). Intra-and interspecific competition by Fopius arisanus and Diachasmimorpha tryoni (Hymenoptera: Braconidae), parasitoids of tephritid fruit flies. Biological Control, 27(3), 251-259.

Zhu, Y. C., & Williams, L. (2002). Detecting the egg parasitoid Anaphes iole (Hymenoptera: Mymaridae) in tarnished plant bug (Heteroptera: Miridae) eggs by using a molecular approach. Annals of the Entomological Society of America, 95(3), 359-365.