INSECTICIDAL AND ANTIOXIDANT ACTIVITIES OF AQUEOUS EXTRACTS OF TWO ALGERIAN MEDICINAL PLANTS
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Feb 10, 2020
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Abstract
In this work, we determined the insecticidal activity and antioxidant activity of aqueous extracts of Nerium oleander (Apocynaceae) leaves and Peganum harmala (Zygophyllaceae) seeds, two well-known Algerian medicinal plants. Four concentrations of each extract were applied in total randomization by spraying directly on the eggs and larvae of Ectomyelois ceratoniae (Zeller, 1879) (Lepidoptera: Pyralidae) under laboratory conditions. Our findings revealed that eggs hatching after four days were not affected by the aqueous extract, with a rate of 54% of hatching eggs in both control and treated eggs. Additionally, toxicity by contact on larvae was not evident and the mortality percentage did not exceed 8%, regardless of the extract plant and concentration. Phytochemical screening showed a slight presence of terpenes and saponins and an absence of alkaloids. However, polyphenols were present in medium concentrations in the aqueous extracts of both plants. The antioxidant activities of N. oleander and P. harmala extracts were found to be 71.80 and 72.94%, respectively. Total phenolic content (TPC) and total flavonoid content (TFC) of the studied extracts were 50.69 mg gallic acid equivalent (GAE)/100 g dry weight (DW) and 3.55 in terms of quercetin equivalent (QE)/100 g DW, respectively, in N. oleander. In P. harmala, the TPC and TFC were 52.30 mg GAE/100 g DW and 3.49 mg QE/100 g DW, respectively. Our study clearly shows the limited insecticidal effect of the aqueous extracts, although they showed an interesting antioxidant potential, which could help to decrease the incidence of oxidative stress-induced damage.
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Nia, B., Lekbir, A., & Ben Salah, M. (2020). INSECTICIDAL AND ANTIOXIDANT ACTIVITIES OF AQUEOUS EXTRACTS OF TWO ALGERIAN MEDICINAL PLANTS. Acta Entomologica Serbica, 25(1), 67-75. https://doi.org/10.5281/zenodo.3660988
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References
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Abolhasani, L., Salehi, E. A., & Kenari, R. E. (2015). Study of antioxidant capacity and stability of phenolic compounds from the seeds of Peganum harmala. Journal of Applied Environmental and Biological Sciences, 4(11s), 218-222.
Akhtar, Y., Yeoung, Y. R., & Isman, M. B. (2008). Comparative bioactivity of selected extracts from Meliaceae and some commercial botanical insecticides against two noctuid caterpillars, Trichoplusia ni and Pseudaletia unipuncta. Phytochemistry Reviews, 7(1), 77-88. http://doi.org/10.1007/s11101-006-9048-7
Bagari, M., Bouhaimi, A., Ghaout, S., & Chihrane, J. (2013). The toxic effects of Nerium oleander on larvae of the desert locust Schistocerca gregaria (Forskål, 1775) (Ortoptera, Acrididae). Zoologica Baetica, 24, 193-203.
Benmehdi, H., Hasnaoui, O., Benali, O., & Salhi, F. (2012). Phytochemical investigation of leaves and fruits extracts of Chamaerops humilis L. Journal of Materials and Environmental Science, 3(2), 320-327.
Camel, V. (2014). Extraction Methodologies: General Introduction. In Encyclopedia of Analytical Chemistry (pp. 1-24). http://doi.org/10.1002/9780470027318.a9902
Cruz-Estrada, A., Gamboa-Angulo, M., Borges-Argáez, R., & Ruiz-Sánchez, E. (2013). Insecticidal effects of plant extracts on immature whitefly Bemisia tabaci Genn. (Hemiptera: Aleyroideae). Electronic Journal of Biotechnology, 16(1), 1-10. http://doi.org/10.2225/vol16-issue1-fulltext-6
Dehghani, M., & Ahmadi, K. (2013). Influence of some plant extracts and commercial insecticides on the eggs of Trialeurodes vaporariorum Westwood (Homoptera: Aleyrodidae). Archives Of Phytopathology And Plant Protection, 46(10), 1127-1135. http://doi.org/10.1080/03235408.2012.760261
Dhawan, D., & Gupta, J. (2016). Comparison of different solvents for phytochemical extraction potential from Datura metel plant leaves. International Journal of Biological Chemistry, 11(1), 17-22. http://doi.org/10.3923/ijbc.2017.17.22
Dixit, A., Singh, H., Sharma, R. A., & Sharma, A. (2015). Estimation of antioxidant and antibacterial activity of crude extracts of Thevetia peruviana (Pers.) K. Schum. International Journal of Pharmacy and Pharmaceutical Sciences, 7(2), 55-59.
Djilani, A., Legseir, B., Soulimani, R., Dickob, A., & Younos, C. (2011). New extraction technique for alkaloids. Journal of the Brazilian Chemical Society, 17(3), 296-305. http://doi.org/10.1145/1966913.1966952
El Allagui, N., Tahrouch, S., Bourijate, M., & Hatimi, A. (2007). Action de différents extraits végétaux sur la mortalité des nématodes à galles du genre Meloidogyne ssp. Acta Botanica Gallica 154(4), 503-509. http://doi.org/10.1080/12538078.2007.10516076
Esmaeili, A. K., Taha, R. M., Mohajer, S., & Banisalam, B. (2015). Antioxidant activity and total phenolic and flavonoid content of various solvent extracts from in vivo and in vitro grown Trifolium pratense L. (Red Clover). BioMed Research International, 11. http://doi.org/10.1155/2015/643285
Farooqui, S., & Tyagi, T. (2018). Nerium oleander: It’s application in basic and applied science: A review. International Journal of Pharmacy and Pharmaceutical Sciences, 10(3), 1. http://doi.org/10.22159/ijpps.2018v10i3.22505
Gursoy, N., Sarikurkcu, C., Cengiz, M., & Solak, M. H. (2009). Antioxidant activities, metal contents, total phenolics and flavonoids of seven Morchella species. Food and Chemical Toxicology, 47(9), 2381-2388. http://doi.org/10.1016/j.fct.2009.06.032
Idder, M. A., Idder-ighili, H., Saggou, H., & Pintureau, B. (2009). Taux d’infestation et morphologie de la pyrale des dattes Ectomyelois ceratoniae (Zeller) sur différentes variétés du palmier dattier Phoenix dactylifera (L.). Cahiers Agricultures, 18(1), 63-71.
Jones, W. P., & Kinghorn, A. D. (2012). Extraction of plant secondary metabolites. In S. D. Sarker & L. Nahar (Eds.), Natural Products Isolation (Springer S, pp. 341-366). Humana Press. http://doi.org/10.1007/978-1-61779-624-1
Juntachote, T., Berghofer, E., Siebenhandl, S., & Bauer, F. (2007). Antioxidative effect of added dried Holy basil and its ethanolic extracts on susceptibility of cooked ground pork to lipid oxidation. Food Chemistry, 100(1), 129-135. http://doi.org/10.1016/j.foodchem.2005.09.033
Kanwal, Z. G., Hafeez, A., Haq, I. U., Rehman, T. U., Aun, S., Shazadi, I., Fatima, N., Rehman, N. U. (2016). Antioxidant, antimicrobial and antileishmanial study of different parts of Peganum harmala. International Journal of Biosciences, 9(1), 45-58. http://doi.org/10.12692/ijb/9.1.45-58
Kaskoos, R. A. (2014). Physico-chemical parameters, phytochemical screening and antioxidant activity of seeds of Peganum harmala collected from Iraq. Asian Journal of Biomedical and Pharmaceutical Sciences, 4(28), 20-24.
Khadhr, M., Bousta, D., Hajaji, H. El, Lachkar, M., Barkai, H., Ibnsouda-Koraichi, S., & Boukhchina, S. (2017). Phytochemical screening, total phenolics and biological activities of Tunisian Peganum harmala seed extracts. Journal of Chemical and Pharmaceutical Research, 9(2), 32-39.
Krishnaveni, M., Madhaiyan, P., Durairaj, S., Amsavalli, L., & Chandrasekar, R. (2013). Antioxidant activity of plants at Chinnatirupathi, Salem, Tamil Nadu, India. International Journal of Pharmaceutical Sciences and Research, 4(10), 3917-3919. http://doi.org/10.13040/IJPSR.0975-8232.4(10).3917-19
Lakhmili, S., Obraim, S., Taourirte, M., Seddiqi, N., & Amraoui, H. (2014). Chemical analysis and antioxidant activity of “Nerium oleander” leaves. OnLine Journal of Biological Sciences, 14(1), 1-7. http://doi.org/10.3844/ojbssp.2014.1.7
Lu, M., Yuan, B., Zeng, M., & Chen, J. (2011). Antioxidant capacity and major phenolic compounds of spices commonly consumed in China. Food Research International, 44, 530-536. http://doi.org/10.1016/j.foodres.2010.10.055
Mansouri, A., Embarek, G., Kokkalou, E., & Kefalas, P. (2005). Phenolic profile and antioxidant activity of the Algerian ripe date palm fruit (Phoenix dactylifera). Food Chemistry, 89(3), 411-420. http://doi.org/10.1016/j.foodchem.2004.02.051
Mohadjerani, M. (2012). Antioxidant activity and total phenolic content of Nerium oleander L. grown in North of Iran. Iranian Journal of Pharmaceutical Research: IJPR, 11(4), 1121-6.
Mokkadem, A. (1999). Cause de Dégradation des plantes médicinales et aromatiques d’Algérie. Revue Vie et Nature, 7, 24-26.
Nia, B., Frah, N., Lekbir, A., & Benhmed, K. (2018). Assessment of toxicity on the basis of total phenolic content in oleander leaves (Nerium oleander L.) against Myzus persicae (Sulzer, 1776) (Hemiptera: Aphididae). Acta Agriculturae Slovenica, 111(2), 265-270. http://doi.org/10.14720/aas.2018.111.2.02.
Peres, L. L. S., Sobreiro, A. I., Couto, I. F. S., Silva, R. M., Pereira, F. F., Heredia-Vieira, S. C., Cardoso, C. A. L., Mauad, M., Scalon, S. P. Q., Verza, S. S., & Mussury, R. M. (2017). Chemical compounds and bioactivity of aqueous extracts of Alibertia spp. in the control of Plutella xylostella L. (Lepidoptera: Plutellidae). Insects, 8(4). http://doi.org/10.3390/insects8040125.
Rababah, T. M., Banat, F., Rababah, A., Ereifej, K., & Yang, W. (2010). Optimization of extraction conditions of total phenolics, antioxidant activities, and anthocyanin of oregano, thyme, terebinth, and pomegranate. Journal of Food Science, 75(7), 626-632. http://doi.org/10.1111/j.1750-3841.2010.01756.x.
Reguieg, L. (2011). Using medicinal plants in Algeria. American Journal of Food and Nutrition, 1(3), 126-127. http://doi.org/10.5251/ajfn.2011.1.3.126.127.
Salari, E., Ahmadi, K., Dehyaghobi, R. Z., Purhematy, A., & Takalloozadeh, H. M. (2012). Toxic and repellent effect of harmal (Peganum harmala L.) acetonic extract on several aphids and Tribolium castaneum (Herbst). Chilean Journal of Agricultural Research, 72(1), 147-151.
Sharififar, F., Dehghn-Nudeh, G., & Mirtajaldini, M. (2009). Major flavonoids with antioxidant activity from Teucrium polium L. Food Chemistry, 112, 885-888. http://doi.org/10.1016/j.foodchem.2008.06.064
Siddiqui, S., Khan, O. Y., Siddiqui, B. S., & Faizi, S. (1987). Analyse de la composition de l’huile de Peganum harmala L. (Zygophyllaceae). Phytochemistry, 26(5), 1548-1550. http://doi.org/10.1016/S0031-9422(00)81861-0
Singh, P., Jayaramaiah, R. H., Sarate, P., Thulasiram, H. V., Kulkarni, M. J., & Giri, A. P. (2014). Insecticidal potential of defense metabolites from Ocimum kilimandscharicum against Helicoverpa armigera. PLoS ONE, 9(8). http://doi.org/10.1371/journal.pone.0104377
Tayoub, G., Tayoub, G., Sulaiman, H., Alorfi, M., & Author, C. (2014). Analysis of oleandrin in oleander extract (Nerium oleander) by HPLC. Journal of Natural Products, 7, 73-78.
Thouri, A., Chahdoura, H., El Arem, A., Omri Hichri, A., Ben Hassin, R., & Achour, L. (2017). Effect of solvents extraction on phytochemical components and biological activities of Tunisian date seeds (var. Korkobbi and Arechti). BMC Complementary and Alternative Medicine, 17(1), 1-10. http://doi.org/10.1186/s12906-017-1751-y
Tobiszewski, M., Tsakovski, S., Simeonov, V., & Namieśnika, J. (2013). Application of multivariate statistics in assessment of green analytical chemistry parameters of analytical methodologies. Green Chem, 15, 1615-1623. http://doi.org/10.1039/C3GC36976E
Vinayagam, A., & Sudha, P. N. (2011). Antioxidant activity of methanolic extracts of leaves and flowers of Nerium indicum. International journal of pharmaceutical sciencse and research, 2(6), 1548-1553.
Abbassi, K., Mergaoui, L., Atay-Kadiri, Z., Ghaout, S., & Stambouli, A. (2005). Biological activities of Peganum harmala (Zygophyllacea) leaves at floral stage on the mortality and reproductive activity of the desert locust. Zoologica Baetica, 16, 31-46.
Abolhasani, L., Salehi, E. A., & Kenari, R. E. (2015). Study of antioxidant capacity and stability of phenolic compounds from the seeds of Peganum harmala. Journal of Applied Environmental and Biological Sciences, 4(11s), 218-222.
Akhtar, Y., Yeoung, Y. R., & Isman, M. B. (2008). Comparative bioactivity of selected extracts from Meliaceae and some commercial botanical insecticides against two noctuid caterpillars, Trichoplusia ni and Pseudaletia unipuncta. Phytochemistry Reviews, 7(1), 77-88. http://doi.org/10.1007/s11101-006-9048-7
Bagari, M., Bouhaimi, A., Ghaout, S., & Chihrane, J. (2013). The toxic effects of Nerium oleander on larvae of the desert locust Schistocerca gregaria (Forskål, 1775) (Ortoptera, Acrididae). Zoologica Baetica, 24, 193-203.
Benmehdi, H., Hasnaoui, O., Benali, O., & Salhi, F. (2012). Phytochemical investigation of leaves and fruits extracts of Chamaerops humilis L. Journal of Materials and Environmental Science, 3(2), 320-327.
Camel, V. (2014). Extraction Methodologies: General Introduction. In Encyclopedia of Analytical Chemistry (pp. 1-24). http://doi.org/10.1002/9780470027318.a9902
Cruz-Estrada, A., Gamboa-Angulo, M., Borges-Argáez, R., & Ruiz-Sánchez, E. (2013). Insecticidal effects of plant extracts on immature whitefly Bemisia tabaci Genn. (Hemiptera: Aleyroideae). Electronic Journal of Biotechnology, 16(1), 1-10. http://doi.org/10.2225/vol16-issue1-fulltext-6
Dehghani, M., & Ahmadi, K. (2013). Influence of some plant extracts and commercial insecticides on the eggs of Trialeurodes vaporariorum Westwood (Homoptera: Aleyrodidae). Archives Of Phytopathology And Plant Protection, 46(10), 1127-1135. http://doi.org/10.1080/03235408.2012.760261
Dhawan, D., & Gupta, J. (2016). Comparison of different solvents for phytochemical extraction potential from Datura metel plant leaves. International Journal of Biological Chemistry, 11(1), 17-22. http://doi.org/10.3923/ijbc.2017.17.22
Dixit, A., Singh, H., Sharma, R. A., & Sharma, A. (2015). Estimation of antioxidant and antibacterial activity of crude extracts of Thevetia peruviana (Pers.) K. Schum. International Journal of Pharmacy and Pharmaceutical Sciences, 7(2), 55-59.
Djilani, A., Legseir, B., Soulimani, R., Dickob, A., & Younos, C. (2011). New extraction technique for alkaloids. Journal of the Brazilian Chemical Society, 17(3), 296-305. http://doi.org/10.1145/1966913.1966952
El Allagui, N., Tahrouch, S., Bourijate, M., & Hatimi, A. (2007). Action de différents extraits végétaux sur la mortalité des nématodes à galles du genre Meloidogyne ssp. Acta Botanica Gallica 154(4), 503-509. http://doi.org/10.1080/12538078.2007.10516076
Esmaeili, A. K., Taha, R. M., Mohajer, S., & Banisalam, B. (2015). Antioxidant activity and total phenolic and flavonoid content of various solvent extracts from in vivo and in vitro grown Trifolium pratense L. (Red Clover). BioMed Research International, 11. http://doi.org/10.1155/2015/643285
Farooqui, S., & Tyagi, T. (2018). Nerium oleander: It’s application in basic and applied science: A review. International Journal of Pharmacy and Pharmaceutical Sciences, 10(3), 1. http://doi.org/10.22159/ijpps.2018v10i3.22505
Gursoy, N., Sarikurkcu, C., Cengiz, M., & Solak, M. H. (2009). Antioxidant activities, metal contents, total phenolics and flavonoids of seven Morchella species. Food and Chemical Toxicology, 47(9), 2381-2388. http://doi.org/10.1016/j.fct.2009.06.032
Idder, M. A., Idder-ighili, H., Saggou, H., & Pintureau, B. (2009). Taux d’infestation et morphologie de la pyrale des dattes Ectomyelois ceratoniae (Zeller) sur différentes variétés du palmier dattier Phoenix dactylifera (L.). Cahiers Agricultures, 18(1), 63-71.
Jones, W. P., & Kinghorn, A. D. (2012). Extraction of plant secondary metabolites. In S. D. Sarker & L. Nahar (Eds.), Natural Products Isolation (Springer S, pp. 341-366). Humana Press. http://doi.org/10.1007/978-1-61779-624-1
Juntachote, T., Berghofer, E., Siebenhandl, S., & Bauer, F. (2007). Antioxidative effect of added dried Holy basil and its ethanolic extracts on susceptibility of cooked ground pork to lipid oxidation. Food Chemistry, 100(1), 129-135. http://doi.org/10.1016/j.foodchem.2005.09.033
Kanwal, Z. G., Hafeez, A., Haq, I. U., Rehman, T. U., Aun, S., Shazadi, I., Fatima, N., Rehman, N. U. (2016). Antioxidant, antimicrobial and antileishmanial study of different parts of Peganum harmala. International Journal of Biosciences, 9(1), 45-58. http://doi.org/10.12692/ijb/9.1.45-58
Kaskoos, R. A. (2014). Physico-chemical parameters, phytochemical screening and antioxidant activity of seeds of Peganum harmala collected from Iraq. Asian Journal of Biomedical and Pharmaceutical Sciences, 4(28), 20-24.
Khadhr, M., Bousta, D., Hajaji, H. El, Lachkar, M., Barkai, H., Ibnsouda-Koraichi, S., & Boukhchina, S. (2017). Phytochemical screening, total phenolics and biological activities of Tunisian Peganum harmala seed extracts. Journal of Chemical and Pharmaceutical Research, 9(2), 32-39.
Krishnaveni, M., Madhaiyan, P., Durairaj, S., Amsavalli, L., & Chandrasekar, R. (2013). Antioxidant activity of plants at Chinnatirupathi, Salem, Tamil Nadu, India. International Journal of Pharmaceutical Sciences and Research, 4(10), 3917-3919. http://doi.org/10.13040/IJPSR.0975-8232.4(10).3917-19
Lakhmili, S., Obraim, S., Taourirte, M., Seddiqi, N., & Amraoui, H. (2014). Chemical analysis and antioxidant activity of “Nerium oleander” leaves. OnLine Journal of Biological Sciences, 14(1), 1-7. http://doi.org/10.3844/ojbssp.2014.1.7
Lu, M., Yuan, B., Zeng, M., & Chen, J. (2011). Antioxidant capacity and major phenolic compounds of spices commonly consumed in China. Food Research International, 44, 530-536. http://doi.org/10.1016/j.foodres.2010.10.055
Mansouri, A., Embarek, G., Kokkalou, E., & Kefalas, P. (2005). Phenolic profile and antioxidant activity of the Algerian ripe date palm fruit (Phoenix dactylifera). Food Chemistry, 89(3), 411-420. http://doi.org/10.1016/j.foodchem.2004.02.051
Mohadjerani, M. (2012). Antioxidant activity and total phenolic content of Nerium oleander L. grown in North of Iran. Iranian Journal of Pharmaceutical Research: IJPR, 11(4), 1121-6.
Mokkadem, A. (1999). Cause de Dégradation des plantes médicinales et aromatiques d’Algérie. Revue Vie et Nature, 7, 24-26.
Nia, B., Frah, N., Lekbir, A., & Benhmed, K. (2018). Assessment of toxicity on the basis of total phenolic content in oleander leaves (Nerium oleander L.) against Myzus persicae (Sulzer, 1776) (Hemiptera: Aphididae). Acta Agriculturae Slovenica, 111(2), 265-270. http://doi.org/10.14720/aas.2018.111.2.02.
Peres, L. L. S., Sobreiro, A. I., Couto, I. F. S., Silva, R. M., Pereira, F. F., Heredia-Vieira, S. C., Cardoso, C. A. L., Mauad, M., Scalon, S. P. Q., Verza, S. S., & Mussury, R. M. (2017). Chemical compounds and bioactivity of aqueous extracts of Alibertia spp. in the control of Plutella xylostella L. (Lepidoptera: Plutellidae). Insects, 8(4). http://doi.org/10.3390/insects8040125.
Rababah, T. M., Banat, F., Rababah, A., Ereifej, K., & Yang, W. (2010). Optimization of extraction conditions of total phenolics, antioxidant activities, and anthocyanin of oregano, thyme, terebinth, and pomegranate. Journal of Food Science, 75(7), 626-632. http://doi.org/10.1111/j.1750-3841.2010.01756.x.
Reguieg, L. (2011). Using medicinal plants in Algeria. American Journal of Food and Nutrition, 1(3), 126-127. http://doi.org/10.5251/ajfn.2011.1.3.126.127.
Salari, E., Ahmadi, K., Dehyaghobi, R. Z., Purhematy, A., & Takalloozadeh, H. M. (2012). Toxic and repellent effect of harmal (Peganum harmala L.) acetonic extract on several aphids and Tribolium castaneum (Herbst). Chilean Journal of Agricultural Research, 72(1), 147-151.
Sharififar, F., Dehghn-Nudeh, G., & Mirtajaldini, M. (2009). Major flavonoids with antioxidant activity from Teucrium polium L. Food Chemistry, 112, 885-888. http://doi.org/10.1016/j.foodchem.2008.06.064
Siddiqui, S., Khan, O. Y., Siddiqui, B. S., & Faizi, S. (1987). Analyse de la composition de l’huile de Peganum harmala L. (Zygophyllaceae). Phytochemistry, 26(5), 1548-1550. http://doi.org/10.1016/S0031-9422(00)81861-0
Singh, P., Jayaramaiah, R. H., Sarate, P., Thulasiram, H. V., Kulkarni, M. J., & Giri, A. P. (2014). Insecticidal potential of defense metabolites from Ocimum kilimandscharicum against Helicoverpa armigera. PLoS ONE, 9(8). http://doi.org/10.1371/journal.pone.0104377
Tayoub, G., Tayoub, G., Sulaiman, H., Alorfi, M., & Author, C. (2014). Analysis of oleandrin in oleander extract (Nerium oleander) by HPLC. Journal of Natural Products, 7, 73-78.
Thouri, A., Chahdoura, H., El Arem, A., Omri Hichri, A., Ben Hassin, R., & Achour, L. (2017). Effect of solvents extraction on phytochemical components and biological activities of Tunisian date seeds (var. Korkobbi and Arechti). BMC Complementary and Alternative Medicine, 17(1), 1-10. http://doi.org/10.1186/s12906-017-1751-y
Tobiszewski, M., Tsakovski, S., Simeonov, V., & Namieśnika, J. (2013). Application of multivariate statistics in assessment of green analytical chemistry parameters of analytical methodologies. Green Chem, 15, 1615-1623. http://doi.org/10.1039/C3GC36976E
Vinayagam, A., & Sudha, P. N. (2011). Antioxidant activity of methanolic extracts of leaves and flowers of Nerium indicum. International journal of pharmaceutical sciencse and research, 2(6), 1548-1553.