THE SUBLETHAL EFFECTS OF FIVE INSECTICIDES ON HABROBRACON HEBETOR SAY PARASITISM ON EPHESTIA KUEHNIELLA ZELLER LARVAE BASED ON PHENOLOXIDASE ENZYME ACTIVITY
Article Sidebar
Published
May 10, 2023
Main Article Content
Abstract
Ephestia kuehniella is an important cereal pest and Habrobracon hebetor is its efficient biocontrol control worldwide. The enzyme phenoloxidase is one of the major components of the insect immune system. This enzyme catalyzes the quinones and other reactive intermediates to reduce parasitoid invasion. In this study, the effects of five commercial insecticides in Iran on the parasitism percentage of H. hebetor and on the activation of phenoloxidase in the hemolymph of host larvae as an important indicator were investigated. The results showed that the percentage of parasitism was significantly lower in insecticide treatments compared to the control (F5, 42= 6.471). There were significant differences in total protein content among the control and insecticides treatments, as well as in the phenoloxidase activity in the host larvae. Accordingly, the highest activity was in the control (0.3924±0092 μM/min/mg protein) and the lowest when larvae were treated with propargite (0.1927±0.0169 μM/min/mg protein). The results confirmed that the insecticides palizin and buprofezin do not have serious negative effects on H. hebetor parasitism when combined in integrated pest management.
Article Details
How to Cite
Asadi, M. (2023). THE SUBLETHAL EFFECTS OF FIVE INSECTICIDES ON HABROBRACON HEBETOR SAY PARASITISM ON EPHESTIA KUEHNIELLA ZELLER LARVAE BASED ON PHENOLOXIDASE ENZYME ACTIVITY. Acta Entomologica Serbica, 28(1), 27-36. https://doi.org/10.5281/zenodo.7919104
Issue
Section
Articles
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
Copyright: © 2019 The ENTOMOLOGICAL SOCIETY OF SERBIA Staff. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
References
Abedi, Z., Saber, M., Gharekhani, G., Mehrvar, A. & Mahdavi, V. (2012). Effects of azadirachtin, cypermethrin, methoxyfenozide and pyridalil on functional response of Habrobracon hebetor Say (Hymenoptera: Braconidae). Journal of Plant Protection Research, 52(3), 353-358.
Abedi, Z., Saber, M., Gharekhani, G., Mehrvar, A., & Kamita, S. G. (2014). Lethal and sublethal effects of azadirachtin and cypermethrin on Habrobracon hebetor (Hymenoptera: Braconidae). Journal of Economic Entomology, 107, 638-645.
Altuntas, H., & Kilic, A. (2010). The effects of parasitism by the ectoparasitoid wasp Bracon hebetor Say (Hymenoptera: Braconidae) on host hemolymph proteins in the Mediterranean flour moth, Ephestia kuehniella Zeller (Lepidoptera: Pyralidae). Turkish Journal of Zoology, 34, 409-416.
Asadi, M., Rafiee-Dastjerdi, H., Nouri-Ganbalani, G., Naseri, B., & Hassanpour, M. (2018). The effects of plant essential oils on the functional response of Habrobracon hebetor Say (Hymenoptera: Braconidae) to its host. Invertebrate Survival Journal, 15, 169-182.
Asadi, M., Rafiee-Dastjerdi, H., Nouri-Ganbalani, G., Naseri, B., & Hassanpour, M. (2019). Insecticidal activity of the isolated essential oils from three medicinal plants on the biological control agent, Habrobracon hebetor Say (Hymenoptera: Braconidae). Acta Biologica Szegediensis, 63(1), 63-68.
Asadi, M., Nouri-Ganbalani, G., Rafiee-Dastjerdi, H., Hassanpour, M., & Naseri, B. (2020). Comparative study about the sublethal effects of chemical and botanical insecticides on the functional response of Habrobracon hebetor Say (Hym.: Braconidae) to larvae of Ephestia kuehniella Zeller (Lep.: Pyralidae). International Journal of Pest Management, 68(1), 80-88.
Asadi, M., Nouri-Ganbalani, G., Rafiee-Dastjerdi, H., Vahedi, H., Hassanpour, M., & Naseri, B. (2021). Effects of plant essential oils on the changes of digestive enzymes in the ectoparasitoid, Habrobracon hebetor Say, with description of its digestive tube. Arthropod-Plant Interactions, 15(6), 929-935.
Asadi, M., Rafiee-Dastjerdi, H., Nouri-Ganbalani, G., Vahedi, H., Naseri, B., & and Hassanpour, M. (2022). The sublethal effects of five commercial insecticides on the amylolytic and proteolytic activity of the biocontrol agent, Habrobracon hebetor Say (Hymenoptera: Braconidae). Acta Entomologica Serbica, 27(2), 55-65.
Azambuja, P. D., Gracia, E. S., & Ratcliffe, N. (1991). Aspects of classification of Hemiptera hemocytes from six triatomine species. Memórias do Instituto Oswaldo, 86, 1-10.
Bradford, M. A. (1976). Rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry, 72, 248-254.
Cerenius, L., & Soderhall, K. (2004). The prophenoloxidase-activating system in invertebrates. Immunology Review, 198, 116-126.
Chase, M. R., Raina, K., Bruno, J., & Sugumaran M. (2000). Purification, characterization and molecular cloning of prophenoloxidase from Sarcophaga bullata. Insect Biochemistry and Molecular Biology, 30, 953-967.
Darwish, E., EL-Shazly, M., & EL-Sherif, H. (2003). The choice of probing site by bracon hebetor Say (Hymenoptera: Braconidae) foraging for Ephestia kuehniella Zeller (Lepidoptera: Pyralidae). Journal of Stored Product Research, 39, 265-276.
Desneux, N., Decourtye, A., & Delpuech, J. M. (2007). The sublethal effects of pesticides on beneficial arthropods. Annual Review of Entomology, 52, 81-106.
Dweck, H. K. M., Gadallah, N. S., & Darwish, E. (2008). Structure and sensory equipment of the ovipositor of Habrobracon hebetor (Say) (Hymenoptera: Braconidae). Micron, 39(8), 255-1266.
Hartzer, K. L., Zhu, K. Y., & Baker, J. E. (2005). Phenoloxidase in Larvae of Plodia interpunctella (Hübner) (Lepidoptera: Pyralidae): molecular cloning of the proenzyme cDNA and enzyme activity in larvae paralyzed and parasitized by Habrobracon hebetor Say (Hymenoptera: Braconidae). Archives of Insect Biochemistry and Physiology, 59, 67-79.
Karimzadeh, J., & Sayyed, A. H. (2011). Immune system challenge in a host parasitoid-pathogen system: interaction between Cotesia plutellae (Kurdjumov) (Hym.: Braconidae) and Bacillus thuringiensis Berliner influences parasitism and phenoloxidase cascade of Plutella xylostella (L.) (Lep.: Plutellidae). Journal of Entomology Society of Iran, 30, 27-38.
Kryukova, N. A., Dubovskiy, I. M., Gryzanova, E. V., Naumkina, E. A., & Glupov, V. V. (2007). Cell immunity response of the Galleria mellonella (L.) (Lepidoptera, Pyralidae) during parasitization of Habrobracon hebetor (Say) (Hymenoptera, Braconidae). Eurasian Entomological Research, 6, 361-364.
Kryukova, N. A., Dubovskiy, I. M., Chertkova, E. A., Vorontsova, L., Slepneva, I. A., & Glupov, V. V. (2011). The effect of Habrobracon hebetor venom on the activity of the prophenoloxidase system, the generation of reactive oxygen species and encapsulation in the hemolymph of Galleria mellonella larvae. Journal of Insect Physiology, 57, 796-800.
Magro, S. R., & Parra, J. R. P. (2001). Biologia do ectoparasitoide Bracon hebetor Say (Hymenoptera: Braconidae) em sete especies de lepidopteros. Science of Agriculture, 58, 693-698.
Mahdavi, V., & Saber, M. (2013). Functional response of Habrobracon hebetor Say (Hymenoptera: Braconidae) to Mediterranean flour moth (Anagasta Kuehniella Zeller) in response to pesticides. Journal of Economic Entomology, 53, 399-403.
Narayan, K. (2004). Insect defense: its impacts on microbial control of insect pest. Current Science, 86, 800-814.
Navaei, A. N., Taghizadeh, M., Javanmoghaddam, H., Oskoo, T., & Attaran, M. R. (2002). Efficiency of parasitoid wasps, Trichogramma pintoii and Habrobracon hebetor against Ostrinia nubilalis and Helicoverpa sp. on maize in Moghan. Proceedings of the 15th Iranian Plant Protection Congress. 7-11 September, Razi University of Kermanshah, Iran.
Rafiee-Dastjerdi, H., Hejazi, M. J., Nouri-Ganbalani, G., & Saber, M. (2008). Toxicity of some biorational and conventional insecticides to cotton bollworm, Helicoverpa armigera (Lepidoptera: Noctuidae) and its ectoparasitoid, Habrobracon hebetor Say (Hymenoptera: Braconidae). Journal of Entomological Society of Iran, 28(1), 27-37.
Sarmadi, S., Nouri-Ganbalani, G., Rafiee-Dastjerdi, H., Hassanpour, M., & Farshbaf-Purabad, R. (2010). The effects of imidacloprid, indoxacarb and deltamethrin on some biological and demographic parameters of Habrobracon hebetor Say (Hymenoptera: Braconidae) in adult stage treatment. Munis Entomology and Zoology, 5, 646-651.
Shehzad, M., Aslam, A., Zain-ul-Abdin, A., Jafir, M., Malik, M. K., Zubair, M., Javed, M. W., & Chaudhary, M. Z. (2019). Biochemical analysis of phenoloxidase in the haemolymph of Galleria mellonella (Lepidoptera, Pyralidae) under natural and artificial envenomation of ectoparasitoid Bracon hebetor Say (Hymenoptera, Braconidae). International Journal of Entomology Research, 4, 92-96.
SPSS. (2004). SPPS for windows. SPSS INC, Illinois.
Taleh, M., Saadati, M., Farshbaf, R., & Khakvar, R. (2014). Partial characterization of phenoloxidase enzyme in the haemocytes of Helicoverpa armigera Hubner (Lepidoptera: Noctuidae). Journal of King Saud University Science, 26, 285-289.
Yu, S. H., Ryo, M. I., Na, J. H., & Chio, W. I. (2003). Effects of host density on egg dispersion and the sex ratio of progeny of Bracon hebetor Say (Hym: Braconidae). Journal of Stored Product Research, 39, 385-393.
Abedi, Z., Saber, M., Gharekhani, G., Mehrvar, A., & Kamita, S. G. (2014). Lethal and sublethal effects of azadirachtin and cypermethrin on Habrobracon hebetor (Hymenoptera: Braconidae). Journal of Economic Entomology, 107, 638-645.
Altuntas, H., & Kilic, A. (2010). The effects of parasitism by the ectoparasitoid wasp Bracon hebetor Say (Hymenoptera: Braconidae) on host hemolymph proteins in the Mediterranean flour moth, Ephestia kuehniella Zeller (Lepidoptera: Pyralidae). Turkish Journal of Zoology, 34, 409-416.
Asadi, M., Rafiee-Dastjerdi, H., Nouri-Ganbalani, G., Naseri, B., & Hassanpour, M. (2018). The effects of plant essential oils on the functional response of Habrobracon hebetor Say (Hymenoptera: Braconidae) to its host. Invertebrate Survival Journal, 15, 169-182.
Asadi, M., Rafiee-Dastjerdi, H., Nouri-Ganbalani, G., Naseri, B., & Hassanpour, M. (2019). Insecticidal activity of the isolated essential oils from three medicinal plants on the biological control agent, Habrobracon hebetor Say (Hymenoptera: Braconidae). Acta Biologica Szegediensis, 63(1), 63-68.
Asadi, M., Nouri-Ganbalani, G., Rafiee-Dastjerdi, H., Hassanpour, M., & Naseri, B. (2020). Comparative study about the sublethal effects of chemical and botanical insecticides on the functional response of Habrobracon hebetor Say (Hym.: Braconidae) to larvae of Ephestia kuehniella Zeller (Lep.: Pyralidae). International Journal of Pest Management, 68(1), 80-88.
Asadi, M., Nouri-Ganbalani, G., Rafiee-Dastjerdi, H., Vahedi, H., Hassanpour, M., & Naseri, B. (2021). Effects of plant essential oils on the changes of digestive enzymes in the ectoparasitoid, Habrobracon hebetor Say, with description of its digestive tube. Arthropod-Plant Interactions, 15(6), 929-935.
Asadi, M., Rafiee-Dastjerdi, H., Nouri-Ganbalani, G., Vahedi, H., Naseri, B., & and Hassanpour, M. (2022). The sublethal effects of five commercial insecticides on the amylolytic and proteolytic activity of the biocontrol agent, Habrobracon hebetor Say (Hymenoptera: Braconidae). Acta Entomologica Serbica, 27(2), 55-65.
Azambuja, P. D., Gracia, E. S., & Ratcliffe, N. (1991). Aspects of classification of Hemiptera hemocytes from six triatomine species. Memórias do Instituto Oswaldo, 86, 1-10.
Bradford, M. A. (1976). Rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry, 72, 248-254.
Cerenius, L., & Soderhall, K. (2004). The prophenoloxidase-activating system in invertebrates. Immunology Review, 198, 116-126.
Chase, M. R., Raina, K., Bruno, J., & Sugumaran M. (2000). Purification, characterization and molecular cloning of prophenoloxidase from Sarcophaga bullata. Insect Biochemistry and Molecular Biology, 30, 953-967.
Darwish, E., EL-Shazly, M., & EL-Sherif, H. (2003). The choice of probing site by bracon hebetor Say (Hymenoptera: Braconidae) foraging for Ephestia kuehniella Zeller (Lepidoptera: Pyralidae). Journal of Stored Product Research, 39, 265-276.
Desneux, N., Decourtye, A., & Delpuech, J. M. (2007). The sublethal effects of pesticides on beneficial arthropods. Annual Review of Entomology, 52, 81-106.
Dweck, H. K. M., Gadallah, N. S., & Darwish, E. (2008). Structure and sensory equipment of the ovipositor of Habrobracon hebetor (Say) (Hymenoptera: Braconidae). Micron, 39(8), 255-1266.
Hartzer, K. L., Zhu, K. Y., & Baker, J. E. (2005). Phenoloxidase in Larvae of Plodia interpunctella (Hübner) (Lepidoptera: Pyralidae): molecular cloning of the proenzyme cDNA and enzyme activity in larvae paralyzed and parasitized by Habrobracon hebetor Say (Hymenoptera: Braconidae). Archives of Insect Biochemistry and Physiology, 59, 67-79.
Karimzadeh, J., & Sayyed, A. H. (2011). Immune system challenge in a host parasitoid-pathogen system: interaction between Cotesia plutellae (Kurdjumov) (Hym.: Braconidae) and Bacillus thuringiensis Berliner influences parasitism and phenoloxidase cascade of Plutella xylostella (L.) (Lep.: Plutellidae). Journal of Entomology Society of Iran, 30, 27-38.
Kryukova, N. A., Dubovskiy, I. M., Gryzanova, E. V., Naumkina, E. A., & Glupov, V. V. (2007). Cell immunity response of the Galleria mellonella (L.) (Lepidoptera, Pyralidae) during parasitization of Habrobracon hebetor (Say) (Hymenoptera, Braconidae). Eurasian Entomological Research, 6, 361-364.
Kryukova, N. A., Dubovskiy, I. M., Chertkova, E. A., Vorontsova, L., Slepneva, I. A., & Glupov, V. V. (2011). The effect of Habrobracon hebetor venom on the activity of the prophenoloxidase system, the generation of reactive oxygen species and encapsulation in the hemolymph of Galleria mellonella larvae. Journal of Insect Physiology, 57, 796-800.
Magro, S. R., & Parra, J. R. P. (2001). Biologia do ectoparasitoide Bracon hebetor Say (Hymenoptera: Braconidae) em sete especies de lepidopteros. Science of Agriculture, 58, 693-698.
Mahdavi, V., & Saber, M. (2013). Functional response of Habrobracon hebetor Say (Hymenoptera: Braconidae) to Mediterranean flour moth (Anagasta Kuehniella Zeller) in response to pesticides. Journal of Economic Entomology, 53, 399-403.
Narayan, K. (2004). Insect defense: its impacts on microbial control of insect pest. Current Science, 86, 800-814.
Navaei, A. N., Taghizadeh, M., Javanmoghaddam, H., Oskoo, T., & Attaran, M. R. (2002). Efficiency of parasitoid wasps, Trichogramma pintoii and Habrobracon hebetor against Ostrinia nubilalis and Helicoverpa sp. on maize in Moghan. Proceedings of the 15th Iranian Plant Protection Congress. 7-11 September, Razi University of Kermanshah, Iran.
Rafiee-Dastjerdi, H., Hejazi, M. J., Nouri-Ganbalani, G., & Saber, M. (2008). Toxicity of some biorational and conventional insecticides to cotton bollworm, Helicoverpa armigera (Lepidoptera: Noctuidae) and its ectoparasitoid, Habrobracon hebetor Say (Hymenoptera: Braconidae). Journal of Entomological Society of Iran, 28(1), 27-37.
Sarmadi, S., Nouri-Ganbalani, G., Rafiee-Dastjerdi, H., Hassanpour, M., & Farshbaf-Purabad, R. (2010). The effects of imidacloprid, indoxacarb and deltamethrin on some biological and demographic parameters of Habrobracon hebetor Say (Hymenoptera: Braconidae) in adult stage treatment. Munis Entomology and Zoology, 5, 646-651.
Shehzad, M., Aslam, A., Zain-ul-Abdin, A., Jafir, M., Malik, M. K., Zubair, M., Javed, M. W., & Chaudhary, M. Z. (2019). Biochemical analysis of phenoloxidase in the haemolymph of Galleria mellonella (Lepidoptera, Pyralidae) under natural and artificial envenomation of ectoparasitoid Bracon hebetor Say (Hymenoptera, Braconidae). International Journal of Entomology Research, 4, 92-96.
SPSS. (2004). SPPS for windows. SPSS INC, Illinois.
Taleh, M., Saadati, M., Farshbaf, R., & Khakvar, R. (2014). Partial characterization of phenoloxidase enzyme in the haemocytes of Helicoverpa armigera Hubner (Lepidoptera: Noctuidae). Journal of King Saud University Science, 26, 285-289.
Yu, S. H., Ryo, M. I., Na, J. H., & Chio, W. I. (2003). Effects of host density on egg dispersion and the sex ratio of progeny of Bracon hebetor Say (Hym: Braconidae). Journal of Stored Product Research, 39, 385-393.