ISOLATION, MOLECULAR IDENTIFICATION, AND PATHOGENICITY OF BEAUVERIA HOPLOCHELI FROM THE PINE PROCESSIONARY MOTH, THAUMETOPOEA PITYOCAMPA (LEPIDOPTERA: NOTODONTIDAE)
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Dec 10, 2024
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Abstract
The pine processionary moth, Thaumetopoea pityocampa (Den. & Schiff.), is a significant defoliator in pine forests. T. pityocampa also causes health risks to people due to their urticating hair. Possible control options include the application of chemical pesticides, mechanical removal, and the use of predator insects. However, these control methods are insufficient to control the pest. Therefore, new environmentally friendly control methods are needed to protect pine forests. In this study, a previously unreported species of entomopathogenic fungus was isolated and identified, and its insecticidal effect against pine processionary moth larvae was determined for the first time. The fungal isolate was identified as Beauveria hoplocheli according to the results of fungal rDNA gene sequence analysis. The insecticidal potential of the fungal isolate was also tested against second-instar larvae of T. pityocampa. The fungal isolate had a high pathogenic effect, ranging from 73.4% to 100% against the T. pityocampa larvae.
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Yaman, M., & Güvendik, T. (2024). ISOLATION, MOLECULAR IDENTIFICATION, AND PATHOGENICITY OF BEAUVERIA HOPLOCHELI FROM THE PINE PROCESSIONARY MOTH, THAUMETOPOEA PITYOCAMPA (LEPIDOPTERA: NOTODONTIDAE). Acta Entomologica Serbica, 29(2). https://doi.org/10.5281/zenodo.14357989
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References
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Barta, M., Horáková, M. K., Georgieva, M., Mirchev, P., Zaemdzhikova, G., Pilarska, D., Takov, D., Hubenov, Z., Pilarski, P., & Georgiev, G. (2019). Entomopathogenic fungi (Ascomycota: Hypocreales) as natural antagonists of the pine processionary Moth Thaumetopoea pityocampa (Denis & Schiffermüller, 1775) (Lepidoptera: Notodontidae) in Bulgaria. Acta Zoologica Bulgarica, 15, 89-96.
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Chelkha, M., Yakkou, L., Houıda, S., Raouane, M., Amghar, S., Campos-Herrera, R., & Hartı, A. (2022). New insights on the impact of earthworm extract on the growth of beneficial soil fungi: species-specific alteration of the nematophagous fungal growth and limitation of an entomopathogenic fungus. Turkish Journal of Zoology, 46, 456-466.
de Boer, J. G., & Harvey, J. A. (2020). Range-expansion in processionary moths and biological control. Insects, 11(5), 267.
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Hajek, A. E., & Goettel, M. S. (2007). Guidelines for evaluating effects of entomopathogens on non-target organisms In: L. A. Lacey & H. K. Kaya (Eds.), "Field Manual of Techniques in Invertebrate Pathology" Dordrecht, Springer Netherlands, 816-833.
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Kanat, M., Alma, H., & Sivrikaya, F. (2005). Effect of defoliation by Thaumetopoea pityocampa (Den. & Schiff.) (Lepidoptera: Thaumetopoeidae) on annual diameter increment of (Pinus brutia Ten.) in Turkey. Annals of Forest Science, 62(1), 91-94.
Kanat, M., & Özbolat, M. (2006). Mass production and release of Calosoma sycophanta L. (Coleoptera: Carabidae) used against the pine processionary moth, Thaumetopoea pityocampa (Schiff.) (Lepidoptera. Thaumetopoediae) in biological control. Turkish Journal of Zoology, 30, 181-185.
Kashian, D. R., & Dodson, S. I. (2002). Effects of common-use pesticides on developmental and reproductive processes in Daphnia. Toxicology and Industrial Health, 18, 225-235.
NeuveÂglise, C., Brygoo, Y., & Riba, G. (1997). 28s rDNA group-I introns: a powerful tool for identifying strains of Beauveria brongniartii. Molecular Ecology, 6(4),373-381.
Ozdemir, I. O., Kushiyev, R., Erper, İ., & Tuncer, C. (2019). Efficacy of entomopathogenic fungi Beauveria bassiana and Metarhizium anisopliae against Thaumetopoea pityocampa Shiff. (Lepidoptera: Thaumatopoeidae). Archives of phytopathology and plant protection, 52(5-6), 470-480.
Robène-Soustrade, I., Jouen, E., Pastou, D., Payet-Hoarau, M., Goble, T., Linderme, D., Lefeuvre, P., Calmès, C., Reynaud, B., Nibouche, S., & Costet, L. (2015). Description and phylogenetic placement of Beauveria hoplocheli sp. nov. used in the biological control of the sugarcane white grub, Hoplochelus marginalis, on Réunion Island. Mycologia, 107(6), 1221-1232.
Rohrlich, C., Merle, I., Mze Hassani, I., Verger, M., Zuin, M., Besse, S., Robène, I., Nibouche, S., & Costet, L. (2018). Variation in physiological host range in three strains of two species of the entomopathogenic fungus Beauveria. PLoS One, 13(7), e0199199.
Sevim, A., Demir, I., & Demirbağ, Z. (2010). Molecular Characterization and virulence of Beauveria spp. from the pine processionary moth, Thaumetopoea pityocampa (Lepidoptera: Thaumetopoeidae). Mycopathologia, 170, 269-277.
Siahmard, O.J., Rhoda Mae, B.P., & Annabelle, U.N. (2017). Molecular Identification of Rhizospheric Fungi Associated with ‘Saba’ Banana via the Amplification of Internal Transcribed Spacer Sequence of 5.8S Ribosomal DNA. Asian Journal of Plant Sciences, 16, 78-86.
Sönmez, E., Demir, İ., Bull, J. C., Butt, T. M., & Demirbağ, Z. (2017). Pine processionary moth (Thaumetopoea pityocampa, Lepidoptera: Thaumetopoeidae) larvae are highly susceptible to the entomopathogenic fungi Metarhizium brunneum and Beauveria bassiana. Biocontrol Science and Technology, 27(10), 1168-1179.
Trematerra, P., Colacci, M., & Sciarretta, A. (2019). Mass trapping trials for the control of pine processionary moth in a pine woodland recreational area. Journal of Applied Entomology, 143(1-2), 129-136.
Yaman, M. (2022). Potential of Entomopathogens in biological control of pine processionary moth (Thaumetopoea Pityocampa (Den. & Schiff.): A Brief Literature Review. ASEAN Journal of Science and Technol-ogy, 3(1), 46-50.
Alkan Akıncı, H., Ozman-Sullivan, S. K., Diler, H., Celik, N., Thomas Sullivan, G., & Karaca, G. (2017). Entomopathogenic fungi isolated from Thaumetopoea pityocampa and their efficacies against its larvae. Fresenius Environmental Bulletin, 26(8), 5251-5257.
Atakan, A. (1991). Biology of harmful insects of Forests in Turkey. Forest General Directorate Publication, Ankara, Turkey.
Aydın, T., Branco, M., Güven, Ö., Gonçalves, H., Lima, A., Karaca, İ., & Butt, T. (2018). Significant mortality of eggs and young larvae of two pine processionary moth species due to the entomopathogenic fungus Metarhizium brunneum. Biocontrol Science and Technology, 28(4), 317-331.
Barta, M., Horáková, M. K., Georgieva, M., Mirchev, P., Zaemdzhikova, G., Pilarska, D., Takov, D., Hubenov, Z., Pilarski, P., & Georgiev, G. (2019). Entomopathogenic fungi (Ascomycota: Hypocreales) as natural antagonists of the pine processionary Moth Thaumetopoea pityocampa (Denis & Schiffermüller, 1775) (Lepidoptera: Notodontidae) in Bulgaria. Acta Zoologica Bulgarica, 15, 89-96.
Bricca, E. S., Nibouche, S., Delatte, H., Normand, F., & Amouroux, P. (2014). Test of the pathogenicity of two commercial Beauveria strains on third-instar larvae of the mango blossom gall midge, Procontarinia mangiferae (Felt) (Diptera: Cecidomyiidae). Fruits, 69(3),18-194.
Cebeci, H. H., Oymen, R. T., & Acer, A. (2010). Control of pine processionary moth, Thaumetopoea pityocampa with Bacillus thuringiensis in Antalya, Turkey. Journal of Environmental Biology, 31, 357-361.
Chelkha, M., Yakkou, L., Houıda, S., Raouane, M., Amghar, S., Campos-Herrera, R., & Hartı, A. (2022). New insights on the impact of earthworm extract on the growth of beneficial soil fungi: species-specific alteration of the nematophagous fungal growth and limitation of an entomopathogenic fungus. Turkish Journal of Zoology, 46, 456-466.
de Boer, J. G., & Harvey, J. A. (2020). Range-expansion in processionary moths and biological control. Insects, 11(5), 267.
Er, M. K., Tunaz, H., & Gokce, A. (2007). Pathogenicity of entomopathogenic fungi to Thaumetopoea pityocampa (Schiff.) (Lepidoptera: Thaumetopoeidae) larvae in laboratory conditions. Journal of Pest Science, 80, 235-239.
Georgieva, M., Mirchev, P., & Georgiev, G. (2020). Syspastospora parasitica (Tul.) Cannon & Hawksw. (Ascomycota: Hypocreales), a parasite of entomopathogenic fungi (Beauveria spp.) attacking pine processionary moth in Bulgaria. Acta Zoologica Bulgarica, 15, 97-102.
Goertz, D., & Hoch, G. (2013). Influence of the forest caterpillar hunter Calosoma sycophanta on the transmission of microsporidia in larvae of the gypsy moth Lymantria dispar. Agricultural and Forest Entomology, 15(2), 178-186.
Hazir, S., Kaya, H., Touray, M., Çimen, H. & Ilan, D. S. (2022). Basic laboratory and field manual for conducting research with the entomopathogenic nematodes, Steinernema and Heterorhabditis, and their bacterial symbionts. Turkish Journal of Zoology, 46(4), 305-350.
Hajek, A. E., & Goettel, M. S. (2007). Guidelines for evaluating effects of entomopathogens on non-target organisms In: L. A. Lacey & H. K. Kaya (Eds.), "Field Manual of Techniques in Invertebrate Pathology" Dordrecht, Springer Netherlands, 816-833.
Hodar, J. A., Castro, J., & Zamora, R. (2003). Pine processionary caterpillar Thaumetopoea pityocampa as a new threat for relict Mediterranean Scots pine forests under climatic warming. Biological Conservation, 110(1), 123-129.
Hyliš, M., Weiser, J., Oborník, M., & Vávra, J. (2005). DNA isolation from museum and type collection slides of microsporidia. Journal of Invertebrate Pathology, 88, 257-260.
Kanat, M., Alma, H., & Sivrikaya, F. (2005). Effect of defoliation by Thaumetopoea pityocampa (Den. & Schiff.) (Lepidoptera: Thaumetopoeidae) on annual diameter increment of (Pinus brutia Ten.) in Turkey. Annals of Forest Science, 62(1), 91-94.
Kanat, M., & Özbolat, M. (2006). Mass production and release of Calosoma sycophanta L. (Coleoptera: Carabidae) used against the pine processionary moth, Thaumetopoea pityocampa (Schiff.) (Lepidoptera. Thaumetopoediae) in biological control. Turkish Journal of Zoology, 30, 181-185.
Kashian, D. R., & Dodson, S. I. (2002). Effects of common-use pesticides on developmental and reproductive processes in Daphnia. Toxicology and Industrial Health, 18, 225-235.
NeuveÂglise, C., Brygoo, Y., & Riba, G. (1997). 28s rDNA group-I introns: a powerful tool for identifying strains of Beauveria brongniartii. Molecular Ecology, 6(4),373-381.
Ozdemir, I. O., Kushiyev, R., Erper, İ., & Tuncer, C. (2019). Efficacy of entomopathogenic fungi Beauveria bassiana and Metarhizium anisopliae against Thaumetopoea pityocampa Shiff. (Lepidoptera: Thaumatopoeidae). Archives of phytopathology and plant protection, 52(5-6), 470-480.
Robène-Soustrade, I., Jouen, E., Pastou, D., Payet-Hoarau, M., Goble, T., Linderme, D., Lefeuvre, P., Calmès, C., Reynaud, B., Nibouche, S., & Costet, L. (2015). Description and phylogenetic placement of Beauveria hoplocheli sp. nov. used in the biological control of the sugarcane white grub, Hoplochelus marginalis, on Réunion Island. Mycologia, 107(6), 1221-1232.
Rohrlich, C., Merle, I., Mze Hassani, I., Verger, M., Zuin, M., Besse, S., Robène, I., Nibouche, S., & Costet, L. (2018). Variation in physiological host range in three strains of two species of the entomopathogenic fungus Beauveria. PLoS One, 13(7), e0199199.
Sevim, A., Demir, I., & Demirbağ, Z. (2010). Molecular Characterization and virulence of Beauveria spp. from the pine processionary moth, Thaumetopoea pityocampa (Lepidoptera: Thaumetopoeidae). Mycopathologia, 170, 269-277.
Siahmard, O.J., Rhoda Mae, B.P., & Annabelle, U.N. (2017). Molecular Identification of Rhizospheric Fungi Associated with ‘Saba’ Banana via the Amplification of Internal Transcribed Spacer Sequence of 5.8S Ribosomal DNA. Asian Journal of Plant Sciences, 16, 78-86.
Sönmez, E., Demir, İ., Bull, J. C., Butt, T. M., & Demirbağ, Z. (2017). Pine processionary moth (Thaumetopoea pityocampa, Lepidoptera: Thaumetopoeidae) larvae are highly susceptible to the entomopathogenic fungi Metarhizium brunneum and Beauveria bassiana. Biocontrol Science and Technology, 27(10), 1168-1179.
Trematerra, P., Colacci, M., & Sciarretta, A. (2019). Mass trapping trials for the control of pine processionary moth in a pine woodland recreational area. Journal of Applied Entomology, 143(1-2), 129-136.
Yaman, M. (2022). Potential of Entomopathogens in biological control of pine processionary moth (Thaumetopoea Pityocampa (Den. & Schiff.): A Brief Literature Review. ASEAN Journal of Science and Technol-ogy, 3(1), 46-50.